Plants were forced to close because truckers blocked two US border crossing points from blocking car parts.
Omar Alghabrabra, Canada’s Transport Minister called it an illegal economic blockade that targets all Canadians.
Trade disruptions are estimated to cost $300m (PS221m per day).
Truckers blocking the Ambassador Bridge were waving Canadian flags and banners. They denounce Prime Minister Justin Trudeau for refusing to remove a rule that required truckers entering Canada to have full coronavirus vaccinations.
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Demonstrators also protested against Covid passports as well as the requirement that they wear masks.
Toyota, the largest car manufacturer in the world, has stopped production at three factories located in Ontario. They stated that no new vehicles would be made there this week.
Also, output was halted at Ford’s engine factory. Stellantis, which is also the owner of Chrysler, stated that parts shortages had affected its Ontario plant.
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General Motors, on the other side of border, said it was forced to cancel two shifts at a Michigan plant where it produces sport utility vehicles.
These shutdowns are a blow to the auto industry, already suffering from a shortage of semiconductor chips worldwide due to the economic effects.
According to industry experts, the protests could lead to company layoffs or increase vehicle prices for consumers.
About 400 trucks are still in Ottawa when the demonstrations started late last month.
Michelle Krebs, a senior analyst at Autotrader in Detroit, stated to AFP that North American assembly plants depended on parts delivery across the Ambassador Bridge.

Trudeau has called the protests “unacceptable”, stating that they have “negatively affected businesses and manufacturers”.
After a week of isolation due to coronavirus, the prime minister returned Monday to parliament
He refused to compromise on federal Covid measures, even though some provinces have begun lifting their restrictions.

His handling of protests has drawn criticism within his party. This is despite the fact that Omicron-related infections are declining significantly.
Candice Bergen, interim Conservative leader, accused Trudeau of wanting a permanent pandemic on Wednesday
Similar protests have been held in Canada, Australia, New Zealand and France. There is a lot of online chatter about a Washington DC trucker protest.